Pacific lamprey (Entosphenus tridentatus) and Western Brook/River lamprey (Lampetra richardsoni) are important ecological and cultural species, yet little is known about their distribution in Puget Sound. They are eaten by everything that eats salmonids and they also provide ocean derived nutrients to watersheds. Lamprey are species of greatest conservation concern in Washington and are at risk of climate change impacts. We are leveraging a concurrent King County (KC) electrofishing project surveying for presence of non-natal juvenile chinook in island streams to compare the ability of less invasive environmental DNA (eDNA) sampling (i.e., water sampling) to detect juvenile chinook and lamprey. We will conduct monthly eDNA surveys at 10 creeks on Vashon Island Feb-May 2026. These months correspond to juvenile chinook outmigration and highest activity for lamprey including spawning time. Our eDNA census will provide the lamprey management unit for Puget Sound important new data on lamprey distribution and aid more effective management. ($15,000)